Journal: Bioinformation
Article Title: Evaluation of in vitro chondrocytic differentiation: A stem cell research initiative at the King Abdulaziz University, Kingdom of Saudi Arabia
doi: 10.6026/97320630014053
Figure Lengend Snippet: Quantitative real time gene expression analysis of the cartilage related genes in hWJSCs cultured in chondrogenic media for 21 days. Increased expression of (A) collagen, type II, alpha 1 [COL2A1], (B) aggrecan [ACAN] and (C) SRY (sex determining region Y)-box 9 [SOX9] was observed compared to controls. The values were expressed as mean ± SEM from 3 experimental replicates. Asterisk (*) indicate statistical significance at p<0.05 compared to control.
Article Snippet: The hWJSCs (2 x104 cells/well) were plated in a 24 well tissue culture plate and exposed to StemPro chondrogenic differentiation basal media (A100071-01, Thermo Fisher Scientific) fortified with chondrogenic supplements (StemPro Kit content) for up to 21 days with fresh changes of media every 72h.
Techniques: Gene Expression, Cell Culture, Expressing, Control